PH9460 | 解钾培养基 硅酸盐细菌培养基 Potassium solubilizing Agar Medium

解钾培养基（又称硅酸盐细菌培养基）在生物实验中主要用于解钾菌（硅酸盐细菌）的筛选、培养及其解钾能力的定量测定。其核心原理是以钾长石粉等难溶性钾矿物为唯一钾源，通过观察菌株能否生长及是否产生“解钾圈”来初筛目标菌株，再结合液体培养和火焰光度法或原子吸收法测定发酵液中可溶性钾的增加量，从而定量评价菌株的解钾效率。

产品用途：用于解钾菌（硅酸盐细菌）的分离和培养研究

培养基配方-含琼脂（g/L）

蔗糖                         5.0g

葡萄糖                       5.0g

硫酸铵                       0.5g

酵母粉                       0.5g

硫酸镁                       0.3g

磷酸氢二钠                   2.0g

硫酸亚铁                    0.03g

硫酸锰                      0.03g

钾长石                       2.0g

Agar（琼脂）                15.0g

pH7.2±0.2（25℃）      

使用说明：称取本品30.4g于1L蒸馏水或去离子水中，加热煮沸1分钟以上使琼脂溶解，搅拌分装，121℃高压灭菌15分钟，灭菌结束后请摇匀，冷至50-55℃，轻轻摇匀，倾注平板，凝固，备用。

培养基配方-不含琼脂（g/L）

蔗糖                         5.0g

葡萄糖                       5.0g

硫酸铵                       0.5g

酵母粉                       0.5g

硫酸镁                       0.3g

磷酸氢二钠                   2.0g

硫酸亚铁                    0.03g

硫酸锰                      0.03g

钾长石                       2.0g

pH7.2±0.2（25℃）                                                   

使用说明：称取本品15.4g于1L蒸馏水或去离子水中，121℃高压灭菌15分钟，备用。

Potassium-solubilizing medium (also known as silicate bacteria medium) is primarily used in biological experiments for the screening, cultivation, and quantitative determination of potassium-solubilizing bacteria (silicate bacteria). The core principle involves using poorly soluble potassium minerals such as potassium feldspar powder as the sole potassium source. Target strains are initially screened by observing whether the bacteria can grow and whether a "potassium-dissolving halo" forms. Subsequently, the efficiency of potassium solubilization is quantitatively evaluated by measuring the increase in soluble potassium in the fermentation broth through liquid culture combined with flame photometry or atomic absorption spectroscopy.

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