| 产品名称 : | PHC009 | Tris-HCl缓冲液(0.5mol/L,pH6.5-8.8) Tris-HCl Buffer (500mM,pH6.5-8.8) |
| 产品品牌 : | 飞净 PHYGENE |
Tris缓冲液(三羟甲基氨基甲烷)是生物实验中应用最广泛的缓冲体系之一,其有效pH范围通常为7.0–9.0,25°C时pKa约8.06,常用于维持核酸、蛋白质及酶反应所需的稳定pH环境。在分子生物学中,TAE(Tris-乙酸-EDTA)和TBE(Tris-硼酸-EDTA)是DNA/RNA琼脂糖凝胶电泳的标准缓冲液,Tris-HCl则广泛用于DNA/RNA溶解、PCR、限制性内切酶/连接酶/聚合酶等酶反应、细胞裂解和质粒提取;在蛋白质研究中,Tris-甘氨酸缓冲液用于SDS-PAGE,Tris-HCl还用于Western blot转膜、抗体稀释及免疫组化/ELISA等。此外,Tris常与EDTA、NaCl、Tween等配制成TE、TBS、TBST等缓冲液,用于核酸保存、蛋白免疫检测和细胞洗涤。使用时需注意Tris的pH受温度影响较大(温度升高pH下降),且对某些酶或细胞可能存在抑制或毒性,应根据具体实验优化浓度和pH。
常温运输与保存,有效期12个月
Tris buffer is one of the most commonly used buffering systems in biological experiments, with an effective pH range of approximately 7.0–9.0, and is used to maintain a stable pH in nucleic acid and protein experiments. In molecular biology, TAE/TBE is used for gel electrophoresis, while Tris-HCl is employed in PCR, enzymatic reactions, and plasmid extraction. In protein research, it is commonly used in SDS-PAGE, Western blotting, and immunoassays. It is also frequently formulated into buffer solutions such as TE, TBS, and TBST. When using these buffers, note that their pH is highly sensitive to temperature changes, so concentrations and pH should be optimized according to experimental requirements.
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