PH9199 | HEPES/4-羟乙基哌嗪乙磺酸/N-(2-羟乙基)哌嗪-N-2-乙烷磺酸 CAS号：7365-45-9

4-羟乙基哌嗪乙磺酸是生物缓冲剂；分离与分析RNA核组分的反应缓冲液、预杂交缓冲液、杂交缓冲液；用于RNA与T4RNA连接酶的3′-终端标记；用在生化诊断试剂盒、DNA/RNA提取试剂盒及PCR诊断试剂盒里。对细胞没有毒性作用。它是一种氢离子缓冲剂，能较长时间控制恒定的pH范围。使用终浓度为10-50mmol/L，一般培养液内含20mmol/LHEPES即可达到缓冲能力。

英文名称：HEPES；N-（2-Hydroxyethyl）Piperazine-N-2-ethanesulfonic acid；4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
其他名称：4-(2-羟乙基)-1-哌嗪乙磺酸；羟乙基哌嗪乙磺酸；N-(2-羟乙基)哌嗪-N'-2-乙烷磺酸；2-[4-(羟乙基)-1-哌嗪基]乙磺酸；N-(2-羟乙基)哌嗪-N'-乙烷磺酸；4-羟乙基哌嗪乙磺酸；2-[4-(2-羟乙基)-1-哌嗪乙烷]磺酸
CAS号：7365-45-9
C8H18N2O4S=238.30
级别：BR
含量：≥99.5%
PH：6.8～8.2
重金属：≤0.0005%
氯化物：≤0.05%
硫酸盐：≤0.005%
熔点：234～238℃
性状：白色结晶粉末。pKa7.5(25℃）。Cood氏系列缓冲剂之一。性稳定。溶于水(2.25mol/L,0℃)。熔点210℃。紫外线吸光度A(5%/1cm,250nm,水中)<0.05
用途：生化研究。配制生化缓冲液。
保存：常温运输与保存，有效期3年

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent; one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES is like water in that its dissociation decreases as the temperature decreases. This makes HEPES a more effective buffering agent for maintaining enzyme structure and function at low temperatures. Lepe-Zuniga et al. reported a phototoxicity of HEPES when exposed to ambient light by the production of hydrogen peroxide, which is not a problem in bicarbonate-based cell culture buffers. It is therefore strongly advised to keep HEPES-containing solutions in darkness as much as possible.