产品名称 : | PH1928 | 嘌呤霉素溶液(Puromycin,10mg/mL)(Puromycin,1mg/mL) |
产品品牌 : | 飞净 PHYGENE |
保存条件: 冰袋运输, -20℃保存,一年有效,尽量避免反复冻融。
Puromycin is an amino nucleoside antibiotic from Streptomyces alboniger, named purinomycin in Chinese, which is often used to screen cells that express pac gene (puror). The pac gene expresses Puromycin N-acetyl-tranferase (Puromycin n-acetyl-tranferase), which, if expressed, induces resistance to purinomycin. This trait is now commonly used to screen stable mammalian cell lines expressing pac. Currently, many commercial lentiviral vectors carry the pac gene (usually labeled as puror on the plasmid map), and cell lines with stable expression of specific genes can be obtained by screening with purinomycin. Purinomycin can also be used to screen Escherichia coli strains and yeast strains that express pac gene. Puromycin can be used not only for screening stable cell lines, but also for maintenance of stable cell lines. Puromycin is characterized by its rapid action on cells, killing 99% of cells that do not express the pac gene within two days.
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