产品名称 : | PH1039 | 甲苯胺蓝染色液(细胞专用) Toluidine Blue Stain Solution, For Cell |
产品品牌 : | 飞净 PHYGENE |
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Toluidine Blue (Toluidine Blue O) is a commonly used synthetic dye, belongs to the quinone imide dye, this kind of dye mainly contains amine group and quinone type benzene ring two hair chromophore, so that the color of the cations in toluidine blue have a dyeing effect, and the acidic substances in the tissue cells are stained by combining with the cations. Toluidine blue also contains two cochromophores, which can promote the dye to ionize into salts, help the chromophores to produce staining force on the tissue, coloring the tissue cells on the section, and staining the nucleus to make it blue; In addition, mast cells contain heterochromatic substances such as heparin and histamine in the cytoplasm, and toluidine blue can be metachromatic purplish red, which is often used for mast cell staining in laboratory research.
Operation steps (for reference only) :
1. Immediately after smear, fix in 95% ethanol for 15s, remove and place on paper towel.
2. Add toluidine blue staining solution (special for cells) for 5min.
3. Add an equal amount of distilled water to the smear, mix it well, and stain it for 15min.
4, wash, dry, mirror inspection. Staining results: Intracellular acidic mucous polysaccharide was red.
温馨提示:1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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